Inhibitory effect of nicotine on bone regeneration in mandibular distraction osteogenesis.
نویسندگان
چکیده
Nicotine is the main chemical component in tobacco products and its effect on bone healing remains controversial. Distraction osteogenesis is an endogenous tissue engineering technique which provides an excellent platform to study bone healing and regeneration. This study aims to evaluate the dose dependent effect of nicotine on bone regeneration using a rabbit model of mandibular distraction osteogenesis. Twenty New Zealand white rabbits were randomly assigned to four groups: sham control, placebo control, low dose nicotine (0.75 g) and high dose nicotine (1.5 g). 60-day time release nicotine pellets or placebo pellets were implanted in the neck subcutaneous tissue of the rabbits one week before osteotomy was performed. Then after three day latency, eleven day active distraction and four week consolidation, the animals were sacrificed and subjected to examinations by radiography, micro-computed tomography and histological analysis. The significantly lower bone volume and appearance of chondrocytes in the high dose nicotine group indicated that the bone regeneration of distraction osteogenesis was compromised by high dose nicotine exposure.
منابع مشابه
Experimental mandibular regeneration by distraction osteogenesis with submerged devices: preliminary results of a canine model.
The authors describe a new technique for reconstruction of mandibular body defects. The feasibility of distraction osteogenesis with submerged (internal) devices for reconstruction of segmental mandibular defects is investigated in an experiment with five adult dogs. A segmental mandibulectomy was performed on the horizontal ramus. The bony defect was regenerated using distraction osteogenesis ...
متن کاملExperimental model of distraction osteogenesis in edentulous rats.
UNLABELLED Distraction osteogenesis (DO) is a surgical technique producing bone lengthening by distraction of the fracture callus. Although a large number of experimental studies on the events associated with DO of craniofacial skeleton have been reported, the few employing rat mandibular bone DO used complicated designs and produced a small volume of newly formed bone. Thus, this study aims to...
متن کاملLocally applied nerve growth factor enhances bone consolidation in a rabbit model of mandibular distraction osteogenesis.
Distraction osteogenesis is widely used in treating deformities, defects, and fractures of both long bones and craniofacial bones. Demands for acceleration of bone consolidation are increased in distraction osteogenesis. Nerve growth factor (NGF) can enhance innervation and bone regeneration in a fracture model and stimulate differentiation of osteoblastic cells. In this study, we tested the ab...
متن کاملDenervation impairs bone regeneration during distraction osteogenesis in rabbit tibia lengthening
BACKGROUND AND PURPOSES The nervous system plays an important role in bone metabolism. However, the effect of denervation on bone formation during distraction osteogenesis (DO) remains unclear. We studied neural influence on bone regeneration during DO in a rabbit model. METHODS 24 New Zealand male white rabbits underwent left tibial osteodistraction. Before distraction, the animals were rand...
متن کاملThe effects of high dose and highly fractionated radiation on distraction osteogenesis in the murine mandible
The ability of irradiated tissue to support bony growth remains poorly defined, although there are anecdotal cases reported showing mixed results for the use of mandibular distraction osteogenesis after radiation for head and neck cancer. Many of these reports lack objective measures that would allow adequate analysis of outcomes or efficacy. The purpose of this experiment was to utilize a rat ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Frontiers in bioscience : a journal and virtual library
دوره 12 شماره
صفحات -
تاریخ انتشار 2007